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. 2022 Apr 28;18(4):e1010496. doi: 10.1371/journal.ppat.1010496

Fig 3. Flangin is part of a stable structure.

Fig 3

(A) Fluorescence Recovery after Photobleaching (FRAP) was performed on the flange of Flangin-mNG cells, Blue circle bleached ROI 01, Red circle non-bleached ROI 02, and Green circle background ROI 03. (B) ROI 01 showed minimal post-bleaching recovery after 12min, n = 4 independent experiments. A lack of fluorescence recovery was also observed in 29 additional FRAP experiments of various lengths and regions of the cell including the small membrane protrusion associated with the posterolateral axonemes. Scale bar = 5 μm. See S3 Movie for confirmation of viability.