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. 2022 Apr 20;110(8):1385–1399.e8. doi: 10.1016/j.neuron.2022.01.012
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit polyclonal anti-Ucn1 Sigma-Aldrich Cat#U4757; RRID: AB_261834
Goat polyclonal anti-CART R&D System Cat#AF163; RRID: AB_2068569
Chicken polyclonal anti-GFP Aves Labs Cat#GFP-1020; RRID: AB_10000240
Goat polyclonal anti-RFP Rockland Cat#200-101-379; RRID: AB_2744552
Rabbit polyclonal anti-mPRβ (PAQR8) Bioss Cat#BS11410R; RRID: N/A
Rabbit polyclonal anti-mPRγ (PAQR5) Abcam Cat#ab236798; RRID: N/A
Rabbit polyclonal anti-Fos Synaptic Systems Cat#226-003; RRID: AB_2231974
Guinea-Pig polyclonal anti-Fos Synaptic Systems Cat#226-004; RRID: AB_2619946
Mouse monoclonal anti-NeuN Millipore Cat#MAB377; RRID: AB_2298772
Rabbit polyclonal anti-PGR Sigma-Aldrich Cat#SAB4502184; RRID: AB_10746273

Bacterial and virus strains

pAAV-flex-taCasp3-TEVp Yang et al., 2013 Addgene_45580
AAV9-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 Chettih and Harvey, 2019 Addgene AAV9; 124603-AAV9
AAV2-Ef1a-DIO-EYFP Karl Deisseroth Lab Addgene AAV2;
27056-AAV2
AAV1-syn-FLEX-jGCaMP7s-WPRE Dana et al., 2019 Addgene AAV1;
104491-AAV1
AAV2-hSyn-DIO-hM3D(Gq)-mCherry Krashes et al., 2011 Addgene AAV2;
44361-AAV2
AAV2-hSyn-DIO-mCherry Bryan Roth Lab Addgene AAV2;
50459-AAV2
AAV2-hSyn-DIO-hM4D(Gi)-mCherry Krashes et al., 2011 Addgene AAV2;
44362-AAV2
AAV1-CMV-SaCAS9-U6-gRNA(Ucn) GeneCopoeia Cat#AA01- MCP001598-AD01-3-200(a,b,c)
AAV1-CMV-SaCAS9-U6-gRNA(CART) GeneCopoeia Cat#217AA01-MCP299483-AD01-3-200
AAV1-CMV-SaCAS9-U6-gRNA(Cck) GeneCopoeia Cat#217AA01-MCP291368-645 AD01-3-200(a,b,c)
AAV1-CMV-SaCAS9-U6-gRNA(Scrambled) GeneCopoeia Cat#217AA01-CCPCTR01-AD01-100

Chemicals, peptides, and recombinant proteins

Progesterone Sigma-Aldrich Cat#5341; CAS: 57-83-0
Clozapine N-oxide Enzo life sciences Cat#BML-NS105-0025; CAS: 34233-69-7

Critical commercial assays

RNAscope Multiplex Fluorescent Reagent Kit v2 ACDbio Cat#320293
Mouse Progesterone ELISA Kit Novus Biological Cat#NBP2-60125-1

Experimental models: Organisms/strains

Mouse: C57BL6/NRj Janvier Labs Cat# 028533
Mouse: B6;129S- Cartpttm1.1(cre)Hze/J The Jackson Laboratory JAX: 028533
Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J The Jackson Laboratory JAX: 007914

Software and algorithms

ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/
Imaris Oxford Instruments https://imaris.oxinst.com
ITK-SNAP Yushkevich P.A. et al., 2006 http://www.itksnap.org
ClearMap2 Kirst C. et al., 2021 and this paper https://github.com/ChristophKirst/ClearMap2https://doi.org/10.5281/zenodo.3924619
TrailMap Friedmann D. et al., 2020 https://github.com/AlbertPun/TRAILMAP
TopMouseTracker This paper https://github.com/Tom-top/TopMouseTrackerhttps://doi.org/10.5281/zenodo.5833515