REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Rabbit polyclonal anti-Ucn1 | Sigma-Aldrich | Cat#U4757; RRID: AB_261834 |
Goat polyclonal anti-CART | R&D System | Cat#AF163; RRID: AB_2068569 |
Chicken polyclonal anti-GFP | Aves Labs | Cat#GFP-1020; RRID: AB_10000240 |
Goat polyclonal anti-RFP | Rockland | Cat#200-101-379; RRID: AB_2744552 |
Rabbit polyclonal anti-mPRβ (PAQR8) | Bioss | Cat#BS11410R; RRID: N/A |
Rabbit polyclonal anti-mPRγ (PAQR5) | Abcam | Cat#ab236798; RRID: N/A |
Rabbit polyclonal anti-Fos | Synaptic Systems | Cat#226-003; RRID: AB_2231974 |
Guinea-Pig polyclonal anti-Fos | Synaptic Systems | Cat#226-004; RRID: AB_2619946 |
Mouse monoclonal anti-NeuN | Millipore | Cat#MAB377; RRID: AB_2298772 |
Rabbit polyclonal anti-PGR | Sigma-Aldrich | Cat#SAB4502184; RRID: AB_10746273 |
Bacterial and virus strains | ||
pAAV-flex-taCasp3-TEVp | Yang et al., 2013 | Addgene_45580 |
AAV9-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 | Chettih and Harvey, 2019 | Addgene AAV9; 124603-AAV9 |
AAV2-Ef1a-DIO-EYFP | Karl Deisseroth Lab | Addgene AAV2; 27056-AAV2 |
AAV1-syn-FLEX-jGCaMP7s-WPRE | Dana et al., 2019 | Addgene AAV1; 104491-AAV1 |
AAV2-hSyn-DIO-hM3D(Gq)-mCherry | Krashes et al., 2011 | Addgene AAV2; 44361-AAV2 |
AAV2-hSyn-DIO-mCherry | Bryan Roth Lab | Addgene AAV2; 50459-AAV2 |
AAV2-hSyn-DIO-hM4D(Gi)-mCherry | Krashes et al., 2011 | Addgene AAV2; 44362-AAV2 |
AAV1-CMV-SaCAS9-U6-gRNA(Ucn) | GeneCopoeia | Cat#AA01- MCP001598-AD01-3-200(a,b,c) |
AAV1-CMV-SaCAS9-U6-gRNA(CART) | GeneCopoeia | Cat#217AA01-MCP299483-AD01-3-200 |
AAV1-CMV-SaCAS9-U6-gRNA(Cck) | GeneCopoeia | Cat#217AA01-MCP291368-645 AD01-3-200(a,b,c) |
AAV1-CMV-SaCAS9-U6-gRNA(Scrambled) | GeneCopoeia | Cat#217AA01-CCPCTR01-AD01-100 |
Chemicals, peptides, and recombinant proteins | ||
Progesterone | Sigma-Aldrich | Cat#5341; CAS: 57-83-0 |
Clozapine N-oxide | Enzo life sciences | Cat#BML-NS105-0025; CAS: 34233-69-7 |
Critical commercial assays | ||
RNAscope Multiplex Fluorescent Reagent Kit v2 | ACDbio | Cat#320293 |
Mouse Progesterone ELISA Kit | Novus Biological | Cat#NBP2-60125-1 |
Experimental models: Organisms/strains | ||
Mouse: C57BL6/NRj | Janvier Labs | Cat# 028533 |
Mouse: B6;129S- Cartpttm1.1(cre)Hze/J | The Jackson Laboratory | JAX: 028533 |
Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | The Jackson Laboratory | JAX: 007914 |
Software and algorithms | ||
ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
Imaris | Oxford Instruments | https://imaris.oxinst.com |
ITK-SNAP | Yushkevich P.A. et al., 2006 | http://www.itksnap.org |
ClearMap2 | Kirst C. et al., 2021 and this paper | https://github.com/ChristophKirst/ClearMap2https://doi.org/10.5281/zenodo.3924619 |
TrailMap | Friedmann D. et al., 2020 | https://github.com/AlbertPun/TRAILMAP |
TopMouseTracker | This paper | https://github.com/Tom-top/TopMouseTrackerhttps://doi.org/10.5281/zenodo.5833515 |