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. 2022 May 9;7(9):e150871. doi: 10.1172/jci.insight.150871

Figure 1. BET inhibition by JQ1 prevents bleomycin-induced skin fibrosis in mice.

Figure 1

(A) Bleomycin-treated mice showed increased dermal thickness and hydroxyproline content in skin, and JQ1 (50 mg/kg) efficiently prevented skin fibrosis in these mice. Original magnification, ×100. (B) Immunofluorescence staining of skin sections showed that JQ1 significantly decreased α-SMA–positive cells (green) in bleomycin-treated mice compared with the vehicle (VEH) group. Similar results were found quantifying F4/80-positive cells (red). Nuclei were stained with DAPI (blue). Original magnification, ×200–400. (C) Acta2, Col1a1, Il6, and Ccl2 were significantly elevated in bleomycin-treated mice and significantly reduced when JQ1 was given. (PBS, n = 8; VEH, n = 12; JQ1, n = 9). Results are expressed as mean ± SD and P < 0.05 was considered significant. Significance was determined by 1-way ANOVA (A and C) and Kruskal-Wallis test (B and C).