Figure 3. B cell–specific disruption of Hem-1 results in impaired peripheral B cell development.

Splenocytes, inguinal LNs, and peritoneal cavity cells were isolated from 6- to 12-week-old WT and Hem1^fl/flMb1Cre mice. Cells were stained with fluorescent conjugated antibodies against the surface markers shown, followed by flow cytometric analyses. (A) Representative flow cytometric dot plot histograms. (B) Bar graphs with quantification of B cell populations isolated from spleens. Each data point represents an individual animal, and the graphs are representative of > 5 independent experiments (n = 22 and n = 20). (C) Representative dot plot histograms (left) and bar graph showing quantification (right) of FO B cells isolated from inguinal LNs (n = 6 and n = 5). Each data point represents an individual animal, and the graph is representative of > 2 independent experiments. (D) Representative contour histograms (left) and bar graph showing quantification of B cell populations isolated from the peritoneal cavity of WT and Hem1^fl/flMb1Cre mice (n = 4 and n = 4). Data represent mean ± SEM and were analyzed via unpaired Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001.