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. 2022 May 5;23(1):378–392. doi: 10.1080/15384047.2022.2041961

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — CAFs-derived EVs transport miR-331-3p to inhibit the expression of SCARA5.

A. PC cells transfected with pCDNA3.1-GFP following co-culture with CAFs transfected with miR-331-3p-Cy3 observed under a laser scanning confocal microscopy. B. miR-331-3p expression in CAFs following miR-331-3p overexpression or inhibition determined by RT-qPCR. C. miR-331-3p expression in EVs isolated from CAFs following miR-331-3p overexpression or inhibition determined by RT-qPCR. D, miR-331-3p expression in SW1990 and PANC-1 cells before and after co-culture with EVs determined by RT-qPCR. E. SCARA5 expression in SW1990 and PANC-1 cells after co-culture with EVs-miR-331-3p or EVs-miR-331-3p-i for 48 h determined by RT-qPCR. F. Expression of SCARA5 in SW1990 and PANC-1 cells after co-culture with EVs-miR-331-3p or EVs-miR-331-3p-i for 48 h determined by Western blot analysis. Experiments were repeated 3 times. ** indicates p < .01, *** indicates p < .001, and **** indicates p < .0001.