Figure 4. Mitotic dry mass loss and dry mass density increase do not require morphological changes.
(A) % of mitotic cells in a wild-type (wt) L1210 population following 5 hr control or S-trityl-L-cysteine (STLC) treatment. N = 4 independent cultures; p-value calculated using Welch’s t-test. (B) Normalized dry mass behavior for control and STLC-treated wt L1210 cells (left) and quantifications of early mitotic dry mass changes (right). N = 31 cells from 19 independent experiments for control; N = 9 cells from 9 independent experiments for STLC; p-value calculated using Welch’s t-test. (C) Same as panel (B), but for dry mass density. (D) Representative phase contrast and mAG-Geminin reporter images of control and Barasertib-treated FUCCI L1210 cells in mitosis. N > 20 cells from three independent experiments. (E) Normalized dry mass behavior for FUCCI L1210 cells treated with indicated chemicals (left) and quantifications of early mitotic dry mass changes (right). N = 36 cells from 13 independent experiments for control; N = 8 cells from eight independent experiments for Barasertib; N = 16 cells from 15 independent experiments for EIPA; p-value calculated using ANOVA. (F) Same as panel (E), but for dry mass density. (G) Normalized intracellular water volume behavior for control and EIPA-treated FUCCI L1210 cells (left) and quantifications of mitotic cell swelling (right). N = 6 cells from five independent experiments for control; N = 8 cells from eight independent experiments for EIPA; p-value calculated using Welch’s t-test. In dry mass, dry mass density, and water volume traces, the thick line and shaded area represent mean ± SD; boxplot line: mean; box: interquartile range; whiskers: 5–95% range. Raw data can be found in Figure 3—source data 1.
Figure 4—figure supplement 1. L1210 cell population dry mass density following mitotic perturbations.
