| Cell line (Mus musculus) |
wt L1210 |
ATCC |
Cat#CCL-219 |
|
| Cell line (Mus musculus) |
FUCCI L1210 |
Other |
|
Generated in a previous study (Son et al., 2015, Nature Methods), cells originate from ATCC (Cat#CCL-219) |
| Cell line (Mus musculus) |
BaF3 |
RIKEN BioResource Center |
Cat#RCB4476 |
|
| Cell line (Homo sapiens) |
S-HeLa |
Other |
|
Kindly provided by laboratory of Kevin Elias from Brigham And Women’s Hospital |
| Cell line (Homo sapiens) |
THP-1 |
Other |
|
Kindly provided by laboratory of Jianzhu Chen from Massachusetts Institute of Technology |
| Cell line (Mus musculus) |
wt L1210 |
ATCC |
Cat#CCL-219 |
|
| Chemical compound, drug |
STLC |
Sigma-Aldrich |
Cat#164739; CAS:2799-07-7 |
|
| Chemical compound, drug |
Barasertib |
Cayman Chemical |
Cat#13600; CAS:639089-54-6 |
Alternative Names: MK 0457, VX 680 |
| Chemical compound, drug |
EIPA |
Cayman Chemical |
Cat#14406; CAS:1154-25-2 |
|
| Chemical compound, drug |
RO-3306 |
Cayman Chemical |
Cat#15149; CAS:872573-93-8 |
All experiments were done using a stock under 2 weeks of age |
| Chemical compound, drug |
Vacuolin-1 |
Cayman Chemical |
Cat#20425; CAS:351986-85-1 |
|
| Chemical compound, drug |
Brefeldin A |
Cayman Chemical |
Cat#11861; CAS:20350-15-6 |
|
| Antibody |
Anti-LAMP-1 conjugated to Alexa Fluor 488 (rat monoclonal) |
Thermo Fisher Scientific |
Cat#53-1071-82; RRID:AB_657536
|
Clone: eBio1D4B (1D4B) (1:50 dilution) |
| Antibody |
IgG2a kappa Isotype Control conjugated to Alexa Fluor 488 (rat monoclonal) |
Thermo Fisher Scientific |
Cat#53-4321-80; RRID:AB_493963
|
Clone: eBR2a (1:50 dilution) |
| Antibody |
Anti-LAMP-1 conjugated to Alexa Fluor 488 (mouse monoclonal) |
Thermo Fisher Scientific |
Cat#53-1079-42; RRID:AB_2016657
|
Clone: eBioH4A3 (1:50 dilution) |
| Other |
RPMI media, powder |
Thermo Fisher Scientific |
Cat#31800022 |
Cell culture media |
| Other |
Heavy water (D2O) |
Sigma-Aldrich |
Cat#151882; CAS:7789-20-0 |
Heavy water used for culture media |
| Other |
NUCLEAR-ID Red DNA stain |
Enzo Life Sciences |
Cat#ENZ-52406 |
DNA stain (1:250 dilution) |
| Other |
10 μm Diameter Duke Standards 2000 Series Uniform Polystyrene Particles |
Thermo Fisher Scientific |
Cat#2010A |
Standard particles for calibration |
| Software, algorithm |
MATLAB R2017b and R2021b |
MathWorks |
|
Used to analyze the SMR raw data and generate data plots |
| Software, algorithm |
Origin 2021b |
OriginLab |
|
Used to perform statistical analyses and generate data plots |
