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. 2022 Apr 19;11:e76021. doi: 10.7554/eLife.76021

Figure 2. Vitamin B12 alleviates dithiothreitol (DTT) toxicity via methionine synthase.

(A) Representative images of wild-type N2 C. elegans after 72 hr of hatching at 20°C on E. coli OP50 diet containing 0, 10, and 10 mM DTT supplemented with 50 nM vitamin B12. Scale bar = 1 mm. (B) Fraction L4 or adult wild-type N2 C. elegans after 72 hr of hatching at 20°C on E. coli OP50 diet containing 0, 10, and 10 mM DTT supplemented with 50 nM vitamin B12. p values are relative to 0 mM DTT. ***p < 0.001 via the t-test. n.s., nonsignificant (n = 3 biological replicates; animals per condition per replicate >80). (C) Representative images of metr-1(ok521) and mmcm-1(ok1637) animals after 72 hr of hatching at 20°C on E. coli OP50 diet containing 0 mM DTT, 10 mM DTT, and 10 mM DTT supplemented with 50 nM vitamin B12. Scale bar = 1 mm. (D) Fraction L4 or adult metr-1(ok521) and mmcm-1(ok1637) animals after 72 hr of hatching at 20°C on E. coli OP50 diet containing 0, 10, and 10 mM DTT supplemented with 50 nM vitamin B12 (n = 3 biological replicates; animals per condition per replicate >60). (E) Representative images of metr-1(ok521) and mmcm-1(ok1637) animals after 72 hr of hatching at 20°C on C. aquatica DA1877 and P. aeruginosa PA14 gacA mutant diets containing either 0 or 10 mM DTT. Scale bar = 1 mm. (F) Fraction L4 or adult metr-1(ok521) and mmcm-1(ok1637) animals after 72 hr of hatching at 20°C on C. aquatica DA1877 and P. aeruginosa PA14 gacA mutant diets containing either 0 or 10 mM DTT. p values are relative to the corresponding 0 mM DTT condition for each mutant. ***p < 0.001 via the t-test. n.s., nonsignificant (n = 3 biological replicates; animals per condition per replicate>60).

Figure 2—source data 1. Vitamin B12 alleviates dithiothreitol (DTT) toxicity via methionine synthase.

Figure 2.

Figure 2—figure supplement 1. Vitamin B12 supplementation reverses dithiothreitol (DTT) toxicity.

Figure 2—figure supplement 1.

(A) Representative images of wild-type N2 C. elegans grown on E. coli OP50 diet containing 10 mM DTT for 72 hr, followed by another 48 hr growth upon supplementation of either water blank (left panel) or 1.5 nmol of vitamin B12 (right panel). Scale bar = 1 mm. (B) Fraction L4 or adult wild-type N2 C. elegans grown on E. coli OP50 diet containing 10 mM DTT for 72 hr, followed by another 48 hr growth upon supplementation of either water blank (10 mM DTT 120 hr) or 1.5 nmol of vitamin B12. ***p < 0.001 via the t-test (n = 3 biological replicates; animals per condition per replicate >60).
Figure 2—figure supplement 1—source data 1. Vitamin B12 supplementation reverses dithiothreitol (DTT) toxicity.