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. 2022 May 10;11:e73405. doi: 10.7554/eLife.73405

Figure 6. Moving trajectories of cells during negative phototaxis.

Figure 6.

(A) Cell images and moving trajectories. Images were integrated with a single image at each time duration presented (see also Videos 12–14). Upper: movement of microcolonies. Left bottom: cell perpendicular to lateral light axis. Right bottom: cell that stood up and kept binding at a cell pole. (B) Cell–cell interaction. The area of a cell moving as a single unit was measured before and after the induction of negative phototaxis and presented as the ratio by the area (N = 353). (C) Moving direction of a cell in relation to the light source. The orientation of single cells during negative phototaxis was measured, and the distribution is presented (N = 148). The absolute angle of the longer axis of a cell was measured in relation to the lateral light axis. The parallel cellular orientation to the light axis (shown on the left) was taken as 0°, whereas the perpendicular orientation (shown on the right) was ideally 90°.

Figure 6—source data 1. Moving trajectories.
Figure 6—source data 2. Area of a cell.
Figure 6—source data 3. Moving direction of a cell in relation to the light source.