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. 2022 May 10;11:e73405. doi: 10.7554/eLife.73405

Table 1. Oligonucleotides used in this study.

Name Sequence (5′–3′) Purpose
pUC19-5R_tvsesA ATGATACGCGGGTGATTACCGAGCTCGAATTCAC sesA
pUC19-6F_tvsesA GTGGCAAAACCACCTTTGCAAGCTTGGCGTAATC
tvsesA-1F_pUC19 GAATTCGAGCTCGGTAATCACCCGCGTATCATTG
tvsesA-2R_Cm TTCTATCAGCTGTCCCATCAATCCCCGAAACTGC
Cm-3F_tvsesA AGTTTCGGGGATTGATGGGACAGCTGATAGAAAC
Cm-4R_tvsesA ATGGGTGATATTGGCTTTTATCAGGCTCTGGGAG
tvsesA-3F_Cm CCCAGAGCCTGATAAAAGCCAATATCACCCATGC
tvsesA-4R_pUC19 TTACGCCAAGCTTGCAAAGGTGGTTTTGCCACAG
pUC19-7R_tvsesB TCAGGGGAGTCCAAAATACCGAGCTCGAATTCAC sesB
pUC19-8F_tvsesB ACGGATTGCCATTGGTTGCAAGCTTGGCGTAATC
tvsesB-1F_pUC19 GAATTCGAGCTCGGTATTTTGGACTCCCCTGATG
tvsesB-2R_Km AGCAGGGGAATTGTTACACAGACTCTTCTGTGAC
Km-5F_tvsesB CACAGAAGAGTCTGTGTAACAATTCCCCTGCTCG
Km-6R_tvsesB GTTCAAGGTCCTCTTGCCAACCTTTCATAGAAGGC
tvsesB-3F_Km TTCTATGAAAGGTTGGCAAGAGGACCTTGAACTG
tvsesB-4R_pUC19 TTACGCCAAGCTTGCAACCAATGGCAATCCGTAG
pUC19-21R_tvcikA AGATGCGATTGTCCATTACCGAGCTCGAATTCAC cikA
pUC19-22F_tvcikA TTCATTGGGCTTACAGTGCAAGCTTGGCGTAATC
tvcikA-1F_pUC19 GAATTCGAGCTCGGTAATGGACAATCGCATCTGC
tvcikA-2R_Cm TTCTATCAGCTGTCCCGCTGATAGCTACTGCTAG
Cm-18F_tvcikA AGCAGTAGCTATCAGCGGGACAGCTGATAGAAAC
Cm-19R_tvcikA AGATATTCCCGCTGTTTTTATCAGGCTCTGGGAG
tvcikA-3F_Cm CCCAGAGCCTGATAAAAACAGCGGGAATATCTGG
tvcikA-4R_pUC19 TTACGCCAAGCTTGCACTGTAAGCCCAATGAACC
pUC19-19R_tvpixD GTTACCATTCGCAAGTTACCGAGCTCGAATTCAC pixD
pUC19-20F_tvpixD GCTAGTCCTGAACCAATGCAAGCTTGGCGTAATC
tvpixD-1F_pUC19 GAATTCGAGCTCGGTAACTTGCGAATGGTAACCG
tvpixD-2R_Cm TTCTATCAGCTGTCCCAATCAGGCGATGTAGTCC
Cm-15F_tvpixD ACTACATCGCCTGATTGGGACAGCTGATAGAAAC
Cm-16R_tvpixD CAGCATTCCCGTAATGTTTATCAGGCTCTGGGAG
tvpixD-3F_Cm CCCAGAGCCTGATAAACATTACGGGAATGCTGTG
tvpixD-4R_pUC19 TTACGCCAAGCTTGCATTGGTTCAGGACTAGCTG
pUC19-23R_tvtll1282 TTTGGCATCTTCAAGGTACCGAGCTCGAATTCAC LOV
pUC19-24F_tvtll1282 TGAGCAAAGGGATCATTGCAAGCTTGGCGTAATC
tvtll1282-1F_pUC19 GAATTCGAGCTCGGTACCTTGAAGATGCCAAAGC
tvtll1282-2R_Cm TTCTATCAGCTGTCCCAGCATAGCGTTCCATCTC
Cm-20F_tvtll1282 GATGGAACGCTATGCTGGGACAGCTGATAGAAAC
Cm-21R_tvtll1282 AATAGGCTCAGGACATTTTATCAGGCTCTGGGAG
tvtll1282-3F_Cm CCCAGAGCCTGATAAAATGTCCTGAGCCTATTGG
tvtll1282-4R_pUC19 TTACGCCAAGCTTGCAATGATCCCTTTGCTCAGC
pUC19-25R_tvHCP TGAACTTTGCCCTTGTTACCGAGCTCGAATTCAC OCP
pUC19-26F_tvCTDH GATAGGGAGATTGCATTGCAAGCTTGGCGTAATC
tvHCP-1F_pUC19 GAATTCGAGCTCGGTAACAAGGGCAAAGTTCAGC
tvHCP-2R_Cm TTCTATCAGCTGTCCCGCTCATCAAAACTGAGGG
Cm-22F_tvHCP CTCAGTTTTGATGAGCGGGACAGCTGATAGAAAC
Cm-23R_tvCTDH TCTTCAAAGGGTGGATTTTATCAGGCTCTGGGAG
tvCTDH-1F_Cm CCCAGAGCCTGATAAAATCCACCCTTTGAAGAGC
tvCTDH-2R_pUC19 TTACGCCAAGCTTGCAATGCAATCTCCCTATCCG
pUC19-15R_tvpilB CAGTTGAATCTGGGTTTACCGAGCTCGAATTCAC pilB
pUC19-16F_tvpilB ACAGGTTTTGGAGGTCTGCAAGCTTGGCGTAATC
tvpilB-1F_pUC19 GAATTCGAGCTCGGTAAACCCAGATTCAACTGGG
tvpilB-2R_Cm TTCTATCAGCTGTCCCTTGTGGTACTGGCGTAAC
Cm-11F_tvpilB TACGCCAGTACCACAAGGGACAGCTGATAGAAAC
Cm-12R_tvpilB AGATCTTGAAGCGGGATTTATCAGGCTCTGGGAG
tvpilB-3F_Cm CCCAGAGCCTGATAAATCCCGCTTCAAGATCTTG
tvpilB-4R_pUC19 TTACGCCAAGCTTGCAGACCTCCAAAACCTGTAC
pUC19-17R_tvpilT1 TTTTTGCCAGCACCTTTACCGAGCTCGAATTCAC pilT1
pUC19-18F_tvpilT1 TCTTGGCTGGCTTGATTGCAAGCTTGGCGTAATC
tvpilT1-1F_pUC19 GAATTCGAGCTCGGTAAAGGTGCTGGCAAAAAGC
tvpilT1-2R_Cm TTCTATCAGCTGTCCCAAGTCTGAACCACCGTTG
Cm-13F_tvpilT1 ACGGTGGTTCAGACTTGGGACAGCTGATAGAAAC
Cm-14R_tvpilT1 TGGCAAGCTGAATCGTTTTATCAGGCTCTGGGAG
tvpilT1-3F_Cm CCCAGAGCCTGATAAAACGATTCAGCTTGCCATC
tvpilT1-4R_pUC19 TTACGCCAAGCTTGCAATCAAGCCAGCCAAGAAG
pUC19-11R_tvpilA1 CTATTCTCTTTGCAGGTACCGAGCTCGAATTCAC pilA1
pUC19-12F_tvpilA1 TGGTTTGGTGCCAGTATGCAAGCTTGGCGTAATC
tvpilA1-1F_pUC19 GAATTCGAGCTCGGTACCTGCAAAGAGAATAGCG
tvpilA1-7R_Cm TTCTATCAGCTGTCCCAGTTGCCGTCTGCTACAG
Cm-32F_tvpilA1 GTAGCAGACGGCAACTGGGACAGCTGATAGAAAC
Cm-6R_tvpilA1 GCAGTTGCACTATTGCTTTATCAGGCTCTGGGAG
tvpilA1-3F_Cm CCCAGAGCCTGATAAAGCAATAGTGCAACTGCTC
tvpilA1-4R_pUC19 TTACGCCAAGCTTGCATACTGGCACCAAACCATG
pUC19-13R_tvHfq CAGCGGGATGTGAATATACCGAGCTCGAATTCAC hfq
pUC19-14F_tvHfq AAATGCGGCTTTCCTATGCAAGCTTGGCGTAATC
tvHfq-1F_pUC19 GAATTCGAGCTCGGTATATTCACATCCCGCTGTG
tvHfq-2R_Cm TTCTATCAGCTGTCCCTACTTGGCGAATGCTAGG
Cm-9F_tvHfq TAGCATTCGCCAAGTAGGGACAGCTGATAGAAAC
Cm-10R_tvHfq ACCTCGGTGCTATCTTTTTATCAGGCTCTGGGAG
tvHfq-3F_Cm CCCAGAGCCTGATAAAAAGATAGCACCGAGGTTG
tvHfq-4R_pUC19 TTACGCCAAGCTTGCATAGGAAAGCCGCATTTGC
pUC19-43R_tvtll1859 ATCCATCGAACAAGCCTACCGAGCTCGAATTCAC tll1859toN
pUC19-44F_tvtll1859 GTCCCGTTCATTGATGTGCAAGCTTGGCGTAATC
tvtll1859-1F_pUC19 GAATTCGAGCTCGGTAGGCTTGTTCGATGGATTG
tvtll1859-2R_Kmv2 cctgagtgcttgcggcTTTCTAGGGGGTGTGAATC
Km-21F_tvtll1859 TCACACCCCCTAGAAAgccgcaagcactcagg
Km-22R_tvtll1859 TTGCAACACAGTTGTCcctttcatagaaggcggc
tvtll1859-3F_Kmv2 cgccttctatgaaaggGACAACTGTGTTGCAAACC
tvtll1859-4R_pUC19 TTACGCCAAGCTTGCACATCAATGAACGGGACTG
pUC19-67F_tvpilA1 gattggtttcaggcaaTGCAAGCTTGGCGTAATC PilA1S114C
pUC19-68R_tvPilA1 aacgatagggagacatTACCGAGCTCGAATTCAC
tvPilA1-8F_pUC19 GAATTCGAGCTCGGTAatgtctccctatcgttctag
TvPilA1-9R_CmR TTCTATCAGCTGTCCCttaagaacagttaggagcag
Cm-44F_TvPilA1 tcctaactgttcttaaGGGACAGCTGATAGAAAC
Cm-45R_TvPilA1 gttaattcctaaggtcTTTATCAGGCTCTGGGAG
TvPilA1-10F_Cm CCCAGAGCCTGATAAAgaccttaggaattaacagag
TvPilA1-11R_pUC19 TTACGCCAAGCTTGCAttgcctgaaaccaatcgg
tvPilA1(S114C)–1F caaacaTGCgctaatgatccccaaggg
tvPilA1(S114C)–2R attagcGCAtgtttgaacataagcatcattc