pUC19-∆tvsesA_Cm |
pUC19-based construct for knockout of sesA gene (NIES2134_109940) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of sesA, resulting in ~2000 bp deletion within ORF; CmR, AmpR
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This study |
pUC19-∆tvsesB_Km |
pUC19-based construct for knockout of sesB gene (NIES2134_119260) containing a kanamycin resistance cassette flanked by ~2500 bp regions upstream and downstream of sesB, resulting in ~2000 bp deletion within ORF; KmR, AmpR
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This study |
pS-∆tlr0911_Sp(F) |
pCR-Script-based construct for knockout of sesC gene (NIES2134_110090) containing a spectinomycin/streptomycin resistance cassette flanked by ~1500 bp regions upstream and downstream of sesC, resulting in ~3400 bp deletion within ORF; SpR/SmR, AmpR
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Enomoto et al., 2015
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pUC19-∆tvcikA_Cm |
pUC19-based construct for knockout of cikA gene (NIES2134_110210) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of cikA, resulting in ~500 bp deletion within ORF; CmR, AmpR
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This study |
pUC19-∆tvpixD_Cm |
pUC19-based construct for knockout of pixD gene (NIES2134_124540) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of pixD, resulting in ~100 bp deletion within ORF; CmR, AmpR
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This study |
pUC19-∆tvtll1282_Cm |
pUC19-based construct for knockout of LOV gene (tll1282; NIES2134_112750) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of cikA, resulting in ~2000 bp deletion within ORF; CmR, AmpR
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This study |
pUC19-∆tvHCPCTDH_Cm |
pUC19-based construct for knockout of OCP genes (HCP; NIES2134_112880 and CTDH; NIES2134_112890) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of OCP, resulting in the deletion of the latter half of HCP ORF, the intergenic region, and the first half of CTDH ORF; CmR, AmpR
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This study |
pUC19-∆tvpilB_Cm |
pUC19-based construct for knockout of pilB gene (NIES2134_124120) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of pilB, resulting in ~600 bp deletion within ORF; CmR, AmpR
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This study |
pUC19-∆tvpilT1_Cm |
pUC19-based construct for knockout of pilT1 gene (NIES2134_124130) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of pilT1, resulting in ~600 bp deletion within ORF; CmR, AmpR
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This study |
pUC19-∆tvpilA1_Cm |
pUC19-based construct for knockout of pilA1 gene (NIES2134_108920) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of pilA1, resulting in ~550 bp deletion within ORF and the promoter region; CmR, AmpR
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This study |
pUC19-∆tvHfq_Cm |
pUC19-based construct for knockout of hfq gene (NIES2134_124130) containing a chloramphenicol resistance cassette flanked by ~2500 bp regions upstream and downstream of hfq, resulting in ~10 bp deletion within ORF; CmR, AmpR
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This study |
pUC19-tvtll1859toN_Kmv2 |
pUC19-based construct for introduction of the frameshift mutation present in WT_N within tll1859 gene (NIES2134_117830) containing a kanamycin resistance cassette inserted after tll1859 ORF, flanked by ~2500 bp regions upstream and downstream of tll1859 gene without any nucleotide deletion; KmR, AmpR
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This study |
pUC19-tvpilA1(S114C)_Cm |
pUC19-based construct for Cys knockin in pilA1 gene (NIES2134_108920) containing a chloramphenicol resistance cassette inserted after pilA1 ORF, flanked by ~2500 bp regions upstream and downstream of pilA1 without any nucleotide deletion; CmR, AmpR
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This study |