Table 1.

Experimental settings and parameters.

SMT method	construct/cell line	TrackIt parameter	value
ITM	halo-SRF in primary hippocampal neurons (figures 1 and 2)	threshold factor	3
		tracking radius	1.8
		min. track length	2
		gap frames	0
		min. track length before gap frame	0
time-lapse microscopy	halo-SRF in primary hippocampal neurons (figure 3)	threshold factor	1.2
		tracking radius	0.564 (continuous)
			1.187 (500 ms TL)
			1.853 (2 s TL)
		min. track length	7 (continuous)
			7 (500 ms TL)
			3 (2 s TL)
		gap frames	0
		min. track length before gap frame	0
TALM	halo-SRF in primary hippocampal neurons (figure 5)	threshold factor	3
		tracking radius	1.8
		min. track length	50
		gap frames	1
		min. track length before gap frame	3
ITM	SNAP, SNAP-MRTF in NIH3T3 cells (figure 6)	threshold factor	3
		tracking radius	1.8
		min. track length	2
		gap frames	0
		min. track length before gap frame	0
ITM	halo-SRF/SNAP-MRTF colocalization (figure 7)	threshold factor	2
		tracking radius	1.8
		min. track length	2
		gap frames	0
		min. track length before gap frame	0