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. 2022 May 5;12(9):e4406. doi: 10.21769/BioProtoc.4406

Table 1. Examples of crystallized VHH-RBD complexes with details of crystallization conditions, the space group, and resolution limit of crystals generated.

Complex RBD-F2 RBD-H3-C1 RBD-C5
Crystal screen PactTM JCSGTM SG1TM
Crystallisation condition 0.1 M SPG, pH 8, 25% Peg 1500 1.0 M Lithium chloride, 0.1 M Citrate pH 4, 20% Peg 6000 0.2 M Na Acetate, 0.1 M Na Cacodylate pH 6.5 and 30% w/v PEG 8000
Time for crystal formation 3 days less than 24 h 3 days optimal growth
Ratio 0.2 μL protein with 0.1 μL reservoir 0.2 μL protein with 0.1 μL reservoir 0.1 μL protein with 0.1 μL reservoir
Concentration 34 mg/mL 18 mg/mL 18 mg/mL
Cryoprotectant glycerol Peg400 glycerol
Data collection
Exposure 0.006s 0.012s 0.008s
Space group P31 P41212 P21212
Resolution ( ) 2.3 1.9 1.5