Table 1. Examples of crystallized VHH-RBD complexes with details of crystallization conditions, the space group, and resolution limit of crystals generated.
Complex | RBD-F2 | RBD-H3-C1 | RBD-C5 |
---|---|---|---|
Crystal screen | PactTM | JCSGTM | SG1TM |
Crystallisation condition | 0.1 M SPG, pH 8, 25% Peg 1500 | 1.0 M Lithium chloride, 0.1 M Citrate pH 4, 20% Peg 6000 | 0.2 M Na Acetate, 0.1 M Na Cacodylate pH 6.5 and 30% w/v PEG 8000 |
Time for crystal formation | 3 days | less than 24 h | 3 days optimal growth |
Ratio | 0.2 μL protein with 0.1 μL reservoir | 0.2 μL protein with 0.1 μL reservoir | 0.1 μL protein with 0.1 μL reservoir |
Concentration | 34 mg/mL | 18 mg/mL | 18 mg/mL |
Cryoprotectant | glycerol | Peg400 | glycerol |
Data collection | |||
Exposure | 0.006s | 0.012s | 0.008s |
Space group | P31 | P41212 | P21212 |
Resolution ( ) | 2.3 | 1.9 | 1.5 |