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. Author manuscript; available in PMC: 2022 May 11.
Published in final edited form as: Nat Methods. 2021 Oct 4;18(10):1259–1264. doi: 10.1038/s41592-021-01279-0

Extended Data Fig. 9 |. AO improves in vivo 3P imaging of hippocampal structures at different depths in the mouse brain, with 1700 nm excitation.

Extended Data Fig. 9 |

a-l, 3P images of neurons in the mouse hippocampus at different depths. a,d,g,j, Lateral and axial images of neurons without and with AO, at 917, 960, 1010, and 1020 μm below dura, respectively. Post-objective powers: 26.5 (a), 10 (d), 27 (g), and 24 mW (j). b,e,h,k, Signal profiles along the green and yellow lines in a, d, g, and j, respectively. c,f,i,l, Corrective wavefront in a, d, g, and j, respectively. For a, a Gad2-IRES-Cre × Ai14 (Rosa26-CAG-LSL-tdTomato) mouse was used; for d, g, and j, neurons in wildtype mice were infected by a mix of AAV-Syn-Cre and AAV-CAG-FLEX-tdTomato. Scale bar, 10 μm. Microscope objective: NA 1.05 25×. Representative results from 9 fields of view and 2 mice.