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. Author manuscript; available in PMC: 2022 May 11.
Published in final edited form as: Nat Methods. 2021 Oct 4;18(10):1259–1264. doi: 10.1038/s41592-021-01279-0

Extended Data Fig. 2 |. Schematics of AO 2P and 3P fluorescence microscopes, and example system correction.

Extended Data Fig. 2 |

a,b, Components of AO 2P and 3P fluorescence microscopes, respectively. DM, deformable mirror; SLM, spatial light modulator (used to introduce artificial aberration); L, lenses; X and Y, galvanometers; PMT, photomultiplier tube. c, Lateral and axial 3P images of a 1-μm-diameter red fluorescent bead, under 1300 nm excitation, taken without and with AO. Post-objective power: 0.13 mW. d, Signal profiles along the purple and yellow lines in c. e, Corrective wavefront applied to the DM. Scale bar, 1 μm. Microscope objective: NA 1.05 25×. Data representative of n>20 bead images taken during n>20 imaging sessions.