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. Author manuscript; available in PMC: 2022 May 11.
Published in final edited form as: Nat Methods. 2021 Oct 4;18(10):1259–1264. doi: 10.1038/s41592-021-01279-0

Extended Data Fig. 5 |. AO improves 3P imaging of beads in a capillary tube.

Extended Data Fig. 5 |

a, Schematics of sample geometry of 1-μm-diameter fluorescent beads in an air-filled capillary tube. b, Lateral and axial (along red dotted line) images of beads without and with AO (phase modulation). Post-objective power: 0.13 mW. Digital gains were applied to No AO images to increase visibility. c,d, 3P signal improvement (AO/No AO) and axial full width at half maximum (FWHM) of a representative bead (white arrowhead in b) as a function of the iteration #, respectively. e, Corrective wavefront, unwrapped (modulo-2π) for visualization purposes. Scale bar, 5 μm. Microscope objective: NA 1.05 25×. Representative results from 2 imaging sessions.