Fig. 3. Autophagy inhibitor (CQ and 3-MA) abated the ability of proliferation and the expression of ATGs induced by bufalin in LoVo cells. (A) The expression of Atg3, Atg5, Beclin 1 and Atg12 was detected by Western blot, being treated with bufalin and 3-MA. (B) The ability of LoVo cell proliferation was detected by CCK-8 being treated with bufalin and 3-MA. (C) The expression of Atg3, Atg5, Beclin 1 and Atg12 was detected by Western blot being treated with bufalin and CQ. (D) The ability of LoVo cell proliferation was detected by CCK-8. (*P < 0.05, compared with control), (#P < 0.05, compared with bufalin group).
