Fig. 5. Immunohistochemical detection of LC3 protein in tumor xenograft samples. H&E staining (A) and immunohistochemistry (B) were performed after the intratumoral injection of 0 mg per kg per day, 0.5 mg per kg per day and 1.0 mg per kg per day of bufalin for 17 days. Immunodetectable LC3 protein is indicated by brown staining; nuclei are counterstained blue. Original magnification, ×200.