Fig. 2.

β-OHB enhanced cardiomyocyte death under hypoxic conditions. a, b Live (green) or dead (red) CMs under normoxia or 12 h of hypoxia in the different dose of β-OHB-treated environment (n = 8, different fields), c CCK-8 cell survival detection of CMs under normoxia or 12 h of hypoxia in the different dose of β-OHB-treated environment (n = 8–12 wells), d LDH release in the culture media under normoxia or 12 h of hypoxia in the different dose of β-OHB-treated environment (n = 8–12 wells). Data are mean ± SEM. **P < 0.01, ****P < 0.0001 vs. hypoxia 0 h. Scale bars, 50 μm