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. 2022 May 10;5:433. doi: 10.1038/s42003-022-03400-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a HEK293 cells stably expressing mouse cGAS fused with a C-terminal NLS (mcGAS-NLS) or the LL/RK-NLS mutant were fractionated and blotted as indicated. C1QBP: membrane marker; α-tubulin: cytosolic marker; H3: nuclear marker. b Schematic of the doxycycline (Dox)-induced mcGAS and LL/RK-NLS constructs. TRE: Tet Response Element; Puro: puromycin; T2A: Thosea asigna virus 2A-like peptide. c HEK293 cells stably expressing mcGAS were treated with 2 μg/mL Dox for 24 h. Cells were harvested at the indicated times after Dox removal. Cell lysates were blotted as indicated. d Stable cGAS knockout RAW264.7 cells reconstituted with the inducible mcGAS or the LL/RK-NLS mutant were treated with 2 μg/mL Dox for 24 h. Then cells were fractionated into five fractions and blotted as indicated. STING: membrane marker; α-tubulin: cytosolic marker; H3: nuclear marker.