Table 2.

Transcriptional phenotypes of hotspot mutations in the proline domain and adjacent domain.

Position	WT amino acid	MT amino acid	Mutation count	Lowest transcription factor activity (% of WT Function)
49	D	H	8	9.7
49	D	N	8	86.9
49	D	V	3	41
49	D	Y	3	96.2
72	P	A	8	44
72	P	S	7	40.2
72	P	H	3	60.6
72	P	T	1	39.8
73	V	M	5	46.7
73	V	L	4	45.4
73	V	E	1	46.4
82	P	L	16	32.8
82	P	T	1	74.9
82	P	S	1	49.1
84	A	V	9	46
84	A	G	4	64.3
89	P	S	7	48
89	P	L	3	49.8
98	P	S	17	8
98	P	L	11	11.7
98	P	T	1	0
98	P	R	1	7.6

All observed missense mutations observed in the proline domain and adjacent domain are annotated with their mutation prevalence and their weakest transcriptional phenotype, defined as the minimum p53 response element transactivation activity as defined in reference [25].