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. 2021 Nov 24;29(5):1042–1054. doi: 10.1038/s41418-021-00902-z

Fig. 4. Mkk6 phosphorylates Gatad2b at S487 and T490.

Fig. 4

A In vitro kinase assay showing that Mkk6 and Mkk3 phosphorylate Gatad2b. B Mass spectrometric analysis of Gatad2b phosphorylation at S487 and T490. C S487 and T490 mutation of Gatad2b (STAA) abolishes its phosphorylation by Mkk6. Quantitative analysis of phosphorylated Gatad2b to total Gatad2b was shown. Data were from three independent experiments and were shown as mean ± SD. ***p ≤ 0.001. D In vitro kinase assay showing that Mkk6 phosphorylates Gatad2b, but not STAA.