Skip to main content
. 2021 Nov 24;29(5):1042–1054. doi: 10.1038/s41418-021-00902-z

Fig. 5. Mkk6 increases histone acetylation through phosphorylation of Gatad2b.

Fig. 5

A Western blot analysis of H3K9ac, H3K27ac and H4K16ac in MEFs infected with SKO plus Flag, Mkk6 or its mutants. B Western blot analysis of H3K9ac, H3K27ac and H4K16ac in MEFs infected with SKO plus shLuc or shGatad2b in the presence or absence of Mkk6. C Tag density pileups of H3K9ac peaks at the indicated time points in MEFs transduced with SKO + Flag, SKO + Mkk6, SKO + AA, SKO + shLuc, SKO + shGatad2b and SKO + shGatad2b + Mkk6. D Number of genes with hyperacetylated promoter in MEFs infected with SKO + Mkk6 and SKO + AA, compared with SKO + Flag control. Venn diagram depicting overlap between them was shown. E Number of genes with hyperacetylated promoter in MEFs infected with SKO + Mkk6 and SKO + Mkk6 + shGatad2b, compared with SKO + Flag or SKO + shLuc control separately. Venn diagram depicting overlap between them was shown. F ChIP-qPCR analysis of H3K9ac, H3K27ac and H4K16ac in promoter regions of Oct4, Sox2 and Nanog in MEFs infected with SKO plus Flag, Mkk6 or its mutants. Data were from three independent experiments and were shown as mean ± SD. **p ≤ 0.01. G ChIP-qPCR analysis of H3K9ac, H3K27ac and H4K16ac in promoter regions of Oct4, Sox2 and Nanog in MEFs infected with SKO plus shLuc or shGatad2b in the presence or absence of Mkk6. Data were from three independent experiments and were shown as mean ± SD. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.