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. 2022 May 11;7:155. doi: 10.1038/s41392-022-00957-5

Fig. 2.

Fig. 2

Klotho favors skeletal system development by promoting osteogenic differentiation. a Venn diagram showing 510 genes uniquely upregulated while 432 genes downregulated in Klotho-ablated mandibles (p-value < 0.05). n = 3. b Heatmap of representative genes associated with osteogenesis. Red indicates upregulated expression; blue indicates downregulated expression. c Gene ontology (GO) enrichment analysis of downregulated genes in total differentially expressed genes (DEGs). d Alkaline phosphatase (ALP) staining and alizarin red staining (ARS), respectively, at 7 days (7d) and 14 days of osteogenic induction in primary calvarial osteoblasts from OsxCre and OsxCre;KLfl/fl mice. n = 3. e Transcription of Osx, Runx2, Alp, Dmp1, Col1α1, and Rankl after 14d induction. n = 3. f ALP staining and ARS after transfected lentivirus particles to overexpress Klotho (OE) or empty load (NC). n = 3. g Transcription of genes after 14 days of induction. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. All data are shown as the mean ± SEM