KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-PIWIL4 | Invitrogen | Cat# PA5–31448, RRID AB_2548922 |
| Rabbit anti-UTF1 | Abcam | Cat# ab105090, RRID:AB_10903244 |
| Rabbit anti-DDX4 | Abcam | Cat# ab13840, RRID:AB_443012 |
| Mouse anti-TIMP1 [2A5] | Abcam | Cat# ab2464, RRID:AB_303083 |
| Rabbit anti-SOX9 | Abcam | Cat# ab185966, RRID:AB_2728660 |
| Rabbit anti-HMGCS2 | Sigma-Aldrich | Cat# HPA027423, RRID:AB_2672266 |
| Mouse anti-PTCH; clone OTI5C7 | Thermo Fisher Scientific | Cat# MA5–24893, RRID:AB_2725144 |
| Rabbit anti-CYP11A1 | Sigma-Aldrich | Cat# HPA016436, RRID:AB_1847423 |
| Mouse anti-p21 | Innovative Research | Cat# 33–7000, RRID:AB_87086 |
| Rabbit anti-ACTA2 | Abcam | Cat# ab5694, RRID:AB_2223021 |
| Rabbit anti-Collagen I | Abcam | Cat# ab34710, RRID:AB_731684 |
| Rabbit anti-Laminin | Abcam | Cat# ab11575, RRID:AB_298179 |
| Rabbit anti-PSAT1 | Proteintech | Cat# 10501–1-AP, RRID:AB_2172597 |
| Rabbit anti-IDH1 | Thermo Fisher Scientific | Cat# PA5–28206, RRID:AB_2545682 |
| Donkey anti-mouse Alexa Flour 488 | Invitrogen | Cat# A21202, RRID:AB_141607 |
| Donkey anti-rabbit Alexa Flour 594 | Invitrogen | Cat# A21207, RRID:AB_141637 |
| Goat anti-rabbit Alexa Flour 488 | Invitrogen | Cat# A32731, RRID:AB_2633280 |
| Rabbit anti-Histone H3 | Abcam | Cat# ab1791, RRID:AB_302613 |
| Biological samples | ||
| Human testis tissue | DonorConnect | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| hCG | EmpowerR pharmacy | N/A |
| Busulfan | Sigma-Aldrich | Cat# 55-98-1 |
| Critical commercial assays | ||
| Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 | 10 X genomics | Cat# PN-1000121 |
| Chromium Next GEM Chip G Single Cell Kit | 10 X genomics | Cat# PN-1000127 |
| Deposited data | ||
| Raw data files and processed data files for scRNA-seq | This paper | GEO: GSE182786 |
| Experimental models: Cell lines | ||
| Human testicular peritubular cells | Technical University of Munich | N/A |
| Experimental models: Organisms/strains | ||
| Immune-deficient nude mice (NCr nu/nu) | Taconic | Cat# TAC:ncrnu, RRID:IMSR_TAC:ncrnu |
| Software and algorithms | ||
| Cell Ranger (version:4.0.0) | Zheng et al.,2017 | https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/using/tutorial_ov |
| Seurat (version: 4.0.0) | Satija et al., 2015 | https://satijalab.org/seurat/index.html |
| GSEA (version: 3.0) | Tamayo, et al. 2005, Mootha, Lindgren, et al. 2003 | https://www.gsea-msigdb.org/gsea/index.isp |
| metascape | Zhou et al. 2019 | https://metascape.org/gp/index.html#/main/step1 |
| monocle 2 | Qiu et al., 2017, Trapnell, et al. 2014 | http://cole-trapnell-lab.github.io/monocle-release/docs/ |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| GraphPad Prism 8 | GraphPad Software Inc. | https://www.graphpad.com/scientific-software/prism/ |
| Leica LAS AF Lite | Leica | https://leica-las-af-lite.updatestar.com/ |