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. 2022 May 10;8:257. doi: 10.1038/s41420-022-01026-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Volcano map of differentially expressed genes in microarray data GSE106656. Red represents upregulated genes and green represents downregulated genes with |log₂FC | ≥ 1 and p < 0.05. B, AQP5 expression in 14 gastritis patients and 7 IM patients in microarray data GSE106656. C, H. pylori infection in mice detected by Giemsa staining. D, AQP5 mRNA level in mouse gastric mucosa determined by RT-qPCR (n = 6). E, AQP5 protein level in mouse gastric mucosa determined by immunohistochemistry (n = 3). F, AQP5 protein level in mouse isolated gastric mucosa determined by Western blot analysis (n = 3). G, AQP5 mRNA level in mouse isolated gastric mucosa determined by RT-qPCR (n = 3). *p < 0.05, **p < 0.01. Data were shown as the mean ± standard deviation. Comparisons of data between two groups were analyzed by independent sample t-test. One-way ANOVA was applied for the comparison of data among multiple groups. The data between groups at different time points were compared by repeated measures ANOVA.