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. 2022 May 10;8:257. doi: 10.1038/s41420-022-01026-0

Fig. 6. H. pylori promotes GEC apoptosis and inflammatory response via ASCL1/AQP5/Wnt/β-catenin axis.

Fig. 6

A mRNA levels of ASCL1 and AQP5 in mouse primary GECs after ASCL1 knockdown or XAV939 treatment determined by RT-qPCR. B The activation of Wnt/β-catenin pathway in mouse primary GECs after ASCL1 knockdown or XAV939 treatment detected by TOPFlash luciferase reporter assay. C mRNA levels of TNF-α and IL-1β in mouse primary GECs after ASCL1 knockdown or XAV939 treatment determined by RT-qPCR. D Protein levels of TNF-α and IL-1β in mouse primary GECs after ASCL1 knockdown or XAV939 treatment determined by ELISA. E Apoptosis of mouse primary GECs after ASCL1 knockdown or XAV939 treatment detected by Annexin V/PI staining. *p < 0.05, **p < 0.01. Data were shown as the mean ± standard deviation. Cell experiments were repeated three times independently. One-way ANOVA with Dunnett’s post hoc test was applied for the comparison of data among multiple groups.