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. 2022 Apr;11(4):699–709. doi: 10.21037/tcr-21-2234

Figure 2.

Figure 2

Effects of knockdown of the expression of ECSIT on cell death, p65 and p53 in MDA-MB-231 breast cancer cells. Stable cell lines were generated with a specific shRNA sequence of shRandom or shECSIT. (A) Cell death was detected by immunofluorescence of PI (red) and Hoechst 33342 (blue). Representative images (scale bar =50 µm) and quantification of PI-positive cells are shown (lower panel; n=5). (B) Western blot analysis of cleaved caspase-3 and GAPDH in total lysates. (C) Cells were harvested and the expression of p53 was examined by Western blotting. The quantification of p53 in nuclei are shown (lower panel; n=3). (D) The levels of p65 were examined by Western blotting. The lines were three independently repeated experiments. Statistical significance was calculated for the indicated paired samples with **, P<0.01 and ***, P<0.001. shRandom, negative control; shECSIT, knockdown of ECSIT; PI, Propidium iodide.