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. Author manuscript; available in PMC: 2023 Sep 15.
Published in final edited form as: Hear Res. 2021 Nov 11;423:108376. doi: 10.1016/j.heares.2021.108376

Fig. 3. Voltage-dependent membrane conductance of OHCs expressing WT-, C1-, or 499-prestin.

Fig. 3.

(A) Current-voltage (I-V) relationships determined for OHCs isolated from Slc26a5+/+ (WT), Slc26a5C1/C1 (C1/C1), and Slc26a5499/499 (499/499) mice. Insets show current responses to rectangular command voltage steps from −160 mV to + 120 mV (20 mV step for 200 msec). Vm readings at 0 nA (broken horizontal lines) gave reversal potentials (Erev). (B) A summary of the cell membrane conductance at Erev. Horizontal bars indicate the mean ± SD: 26 ± 13 nS (n=19), 32 ± 17 nS (n=10), and 20 ± 8 nS (n=6) for WT, C1/C1, and 499/499, respectively. These values were statistically indistinguishable (one-way ANOVA followed by the Dunnett’s Multiple Comparison Procedure). (C) The resting membrane potentials of OHCs determined by whole-cell current-clamp (at 0 nA). Horizontal bars indicate the mean ± SD: −61 ± 8 mV (n=19), −58 ± 11 mV (n=10), and −62 ± 5 mV (n=6) for WT, C1/C1, and 499/499, respectively. These values were statistically indistinguishable (one-way ANOVA followed by the Dunnett’s Multiple Comparison Procedure). The ages of mice (postnatal days) were: 25, 33, and 34 for Slc26a5+/+; 24, 25, 28, and 29 for Slc26a5C1/C1; 32, 33, and 34 for Slc26a5499/499.