FIG. 3.

Phylogenetic relationships of strains PS1^T, RB1, E, and W based on dioxygenases, including both bph and nah type dioxygenases. Dioxygenase sequences were aligned by using Pileup (26) and were analyzed by using PAUP, version 3.0s (56). The numbers at major nodes are bootstrap percentages; only values greater than 50% are shown. The sequences in boxes were obtained in this study. C. pugetii PS-1^T is a Puget Sound isolate; strain W is a Gulf of Mexico isolate. “C. oligotrophus” RB1 was isolated from Resurrection Bay, Alaska (5).