Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 10;13:2543. doi: 10.1038/s41467-022-30105-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Screenshot of the RNA Pol II ChIP sequencing (ChIP-seq) signal (GSE188640) at the ITGB1 promoter locus in MDA-MB-231 (231_Pol _II.bw) and 231.KO#1 (KO_Pol_II.bw) cells. b qRT-PCR analysis of ITGB1 mRNA expression in the indicated cells. Data are presented as means ± S.E.M. t-test (two-sided). Three biologically independent experiments. c qRT-PCR analysis of ITGB1 mRNA expression in the indicated cells. Data are presented as means ± S.E.M. t-test (two-sided). Three biologically independent experiments. d ITGB1 promoter activity in MDA-MB-231, 231.sgCtrl, 231.KO#1, and 231.KO#2 cells as measured using a dual-luciferase reporter assay. ITGB1 firefly luciferase signal was divided by the control renilla luciferase signal, and the ratios of luciferase/renilla in four cell lines were normalized to that of MDA-MB-231 cells. n = 6 biologically independent experiments in 231.sgCtrl and 231.KO#1 cells; n = 8 biologically independent experiments in MDA-MB-231 cells, n = 9 biologically independent experiments in 231.KO#2 cells. Data are means ± SEM, t-test (two-sided). e Top: the locations of the primers at the ITGB1 gene promoter area for ChIP-qPCR. TSS, transcription start site. Bottom: EZH2 was immunoprecipitated from MDA-MB-231 and 231.KO#1 cells, and EZH2 binding to ITGB1 in the cells was detected using qPCR with the indicated primers. All fold-enrichment values were normalized according to IgG values. Three biologically independent experiments. Data are presented as means ± S.E.M. t-test (two-sided). f RNA Pol II was immunoprecipitated from MDA-MB-231 and 231.KO#1 cells, and RNA Pol II binding to ITGB1 in the cells was detected using qPCR with the indicated primers. All fold-enrichment values were normalized according to IgG values. Three biologically independent experiments. Data are presented as means ± S.E.M. t-test (two-sided). g RNA Pol II was immunoprecipitated from 231.shScr, 231.shEZH2#3, and 231.shEZH2#4 cells, and RNA Pol II binding to ITGB1 or HOXA9B in the cells was detected using qPCR with the indicated primers. HOXA9B was used as a negative control. All fold-enrichment values were normalized according to IgG values. Three biologically independent experiments using P1-P5 primers; six biologically independent experiments using HOXA9B primer. Data are presented as means ± S.E.M. t-test (two-sided). All P values are indicated in the figures.