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High resolution imaging of CAM tumor sections shows that hyaluronan-rich trails are formed in tumors in ovo. Confocal 3D projections from CAM tumor paraffin sections (A, B) derived from MCF10CA cells show high amount of hyaluronan between and around tumor cells and hyaluronan-rich trails (arrowheads in A, B). TEM images (C–F) show the numerous EVs of variable size and shape in the stromal areas (arrows in C–F). Asterisks (*) in (D, E) indicate collagen bundles.
