Fig. 6.

Hemorrhage of r-hirudin delivery via 3DMN and SC. (A) Administration of r-hirudin via MNP and SC injection on the abdomen skin of SD rats. (B) Administration of r-hirudin via MNP and subcutaneous injection on C57BL/6 abdomen skin daily for three days (methylene blue was added to the fabrication process of r-hirudin loaded MNs for better observation ). (C) Representative images of intracutaneous ecchymosis after continuous administration of r-hirudin via MNP and SC every half hour for three times.