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. 2022 Feb 25;17(2):253–267. doi: 10.1016/j.ajps.2022.01.002

Fig. 5.

Fig 5

Cytotoxicity and apoptosis investigation. (A) In vitro cytotoxicity assays of free ICG, ZnO2@Lip, ICG@Lip and ZnO2@Lip-ICG with or without 808 nm laser irradiation (1.0 W/cm2) at pH 7.4 or 6.5 (n = 3); (B) The apoptosis of CNE-2 cells after various treatments was detected by calcein AM/PI staining (Green: live cells, Red: dead cells. Scale bar: 100 μm.); (C) The expression levels of Bax, Caspase-3, Caspase-9 and Caspase-12 in CNE-2 cells after different treatments (1: Control pH7.4; 2: Control pH6.5; 3: ICG@Lip pH7.4; 4: ICG@Lip pH6.5; 5: ZnO2@Lip-ICG pH 7.4; 6: ZnO2@Lip-ICG pH 6.5; 7: Control+Laser pH 7.4; 8: Control+Laser pH 6.5; 9: ICG@Lip+Laser pH 7.4; 10: ICG@Lip+Laser pH 6.5; 11: ZnO2@Lip-ICG+Laser pH 7.4; 12: ZnO2@Lip-ICG+Laser pH 6.5).