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Purification of gallic acid decarboxylase from P. agglomerans T71a
| Step | Amt of protein (mg) | Total activity (U) | Sp act (U/mg) | Fold purification | Yield (%) |
|---|---|---|---|---|---|
| Crude extract | 814 | 7,420 | 9.1 | 1.0 | 100 |
| (NH4)2SO4 (30–50%) | 305 | 6,120 | 20 | 2.2 | 82 |
| DEAE-Sephacel | 44.8 | 3,380 | 75 | 8.3 | 46 |
| (NH4)2SO4 (40–50%) | 17.3 | 1,810 | 105 | 11.5 | 24 |
| Superdex 16-60 Hi-load | 5.2 | 782 | 150 | 16.5 | 11 |
The original preparation contained 4.2 g (dry weight) of cells.
