Fig. 2.

The consecutive two-month evaluation of the mouse estrous cycle, ovarian morphology and hormone levels after BMSCs^mT/mG transplantation. A An ideograph of BMSCs^mT/mG transplantation by tail intravenous and ovarian local injection. B Representative images of the estrous cycle: a: Proestrus; b: Estrus; c: Metoestrus; d: Diestrus. Scale bar = 100 μm. C The number of estrous cycles between the four groups within two months. **P < 0.01, ***P < 0.001. D The percent of mice with an estrous cycle at more than one time point. *P < 0.05, **P < 0.01, ↑ shows rising tendency from control groups; E Each stage duration of estrous cycle between the four groups; the estrus cycle decreasing with age was increased and prolonged due to BMSCs^mT/mG. *Significantly different from Oc + NS at P < 0.05, ^#Significantly different from Ti + BMSCs at P < 0.05, ^##Significantly different from Ti + BMSCs at P < 0.01. F Ovaries from 36w and 40w mice after four and eight weeks of BMSCs^mT/mG transplantation. Isolated ovaries for Ti + BMSCs vs. Ti + NS and Oc + BMSCs vs. Oc + NS. Scale bar = 10 mm; G The weight index of ovaries isolated from 36w, 38w and 40w mice in Ti + NS, Ti + BMSCs, Oc + NS and Oc + BMSCs groups. H The hormone levels of FSH/LH/AMH/E₂ were measured by CLIA test at 32w, 36w, 38w and 40w of each group. The error bars indicate SD. *P < 0.05; **P < 0.01; ↑ shows rising tendency; ↓ shows downtrend.