Fig. 1.

Baicalin represses proliferation of OS cells. (A) HOS, MG63, U2OS and 143B cells were incubated with different concentrations of baicalin for 24–72 h. Cell viability was quantified using a CCK-8 assay. (B) Colony formation after baicalin treatment was evaluated as determined by the colony formation assay. (C) Baicalin-induced S-phase cell cycle arrest was detected by flow cytometry. (D) Expression of c-Myc, cyclin A2, Cdk2 and cyclinE1was analyzed by western blotting. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control group.