TABLE 1.
Purification of endoglucanases from T. neapolitana NS-E
| Purification step | Total protein (mg) | Total activity (U)a | Sp act (U/mg) | Purifi-cation (fold) | % Recovery |
|---|---|---|---|---|---|
| Cell extract | 2,623 | 9,904 | 3.78 | 1 | 100 |
| pH 4.3 | 687 | 8,391 | 12.2 | 3 | 84.7 |
| CelA | |||||
| FFQ-Sepharose | 90 | 2,968 | 33 | 9 | 30 |
| Bio-Gel P-60 | 4.9 | 2,894 | 593 | 157 | 29.2 |
| Mono-Q | 2.0 | 1,952 | 976 | 258 | 19.7 |
| Preparative PAGE | 1.4 | 1,738 | 1,219 | 322 | 17.5 |
| CelB | |||||
| FFQ-Sepharose | 75 | 4,421 | 59 | 15 | 44.6 |
| Sephadex G-50 | 2.0 | 3,050 | 1,534 | 406 | 30.8 |
| Second Sephadex G-50 | 1.8 | 2,759 | 1,536 | 406 | 27.8 |
a
The substrate used for enzyme assays was CMC.