TABLE 2.
Characteristics of T. neapolitana endoglucanases CelA and CelB
| Enzyme | Molecular mass (kDa) | pI | Optimum pH | Optimum temp (°C) | Half-life (min) at:
|
Sp act (U/mg) with:
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 100°C | 106°C | 110°C | CMC | Amorphous cellulose | Crystalline cellulose | Oat spelt xylan | pNP-cellobioside | pNP-glucoside | pNP-lactoside | Cello-biose | Cello-triose | Cello-tetrose | |||||
| CelA | 29.0 | 4.6 | 6.0 | 95 | NDa | ND | ND | 1,219 | 9.45 | 0.11 | ND | 69.2 | <0.01 | 4.9 | 0.0 | ND | ND |
| CelB | 29.3, 30.2 | 4.1 | 6.0–6.6 | 106 | >240 | 130 | 26 | 1,536 | 38.5 | 0.71 | 37 | 18.4 | <0.18 | 2.6 | 0.0 | 264 | 601 |
ND, not determined.