TABLE 4.
High-performance liquid chromatography analysis of products of hydrolysis of cellooligosaccharides by CelB
| Substrate | Incubation time (min) | Concn of reaction products (mM)
|
|||||
|---|---|---|---|---|---|---|---|
| Glucose | Cellobiose | Cellotriose | Cellotetrose | Cellopentose | Cellohexose | ||
| Cellobiose | 0 | —a | 5 (100)b | — | — | — | — |
| 180 | — | 5 (100) | — | — | — | — | |
| Cellotriose | 0 | — | — | 5 (100) | — | — | — |
| 10 | — | 2.5 (33.3) | 2.3 (46) | 0.4 (10.6) | 0.19 (6.3) | 0.09 (3.6) | |
| 180 | 1.9 (12.6) | 5 (66.6) | 0.96 (19.2) | 0.07 (1.8) | — | — | |
| Cellotetrose | 0 | — | — | 0.1 (1.5) | 4.7 (94) | 0.15 (3.7) | 0.02 (0.6) |
| 10 | — | 3.3 (33) | 1.7 (25.5) | 0.9 (18) | 0.46 (11.5) | 0.4 (12) | |
| 180 | 2.1 (10.5) | 6.2 (62) | 1.6 (24) | 0.2 (4) | — | ||
| Cellopentose | 0 | — | — | — | — | 2.2 (100) | |
| 10 | — | 2.6 (47.2) | 1.1 (30) | 0.4 (14.5) | 0.15 (6.8) | — | |
| 180 | 1.8 (16.3) | 3.6 (65.4) | 0.6 (16.3) | 0.03 (1) | — | — | |
| Cellohexose | 0 | — | — | 0.2 (3.2) | 0.3 (6.3) | — | 2.85 (90.4) |
| 10 | — | 2.1 (22.2) | 1.5 (23.8) | 0.8 (16.9) | 0.5 (13.2) | 0.8 (25.4) | |
| 180 | 1.3 (6.9) | 4.7 (49.7) | 1.5 (23.8) | 0.5 (10.5) | 0.2 (5.3) | 0.06 (1.9) | |
a
—, not detected.
b
The numbers in parentheses are the percentages of carbohydrates calculated as glucose.