FIGURE 2.

Curcumin was confirmed to promote the antioxidant function of HAPI cells. (A) The activity of the HAPI cells treated with curcumin at different concentrations for 12 h was determined, and the cellular activity of the cells was evaluated by the CCK-8 analysis. (B) In vitro experimental procedures. (C,D) Based on the CCK-8 analysis, the heme-induced HAPI cells were treated with curcumin (10 μM) for 12 h, then the levels of MDA and GSH were determined. (E) After ROS in each group were labeled by FITC, flow cytometry results were performed. (F) ROS levels in HAPI cells were measured with a microplate reader. Data are shown as the means ± SD of n = 3 samples/group and at least three independent experiments were performed. Statistical analyses were conducted using two-way ANOVA. *, p < 0.01 and **, p < 0.01.