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. 2022 Apr 27;13:889226. doi: 10.3389/fphar.2022.889226

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Copyright © 2022 Duan, Wang, Jiao, Geng, Wu, Yan and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Curcumin could activate the Nrf2/HO-1 pathway in rat primary microglia. (A) Western blot analysis of each group of primary microglia. (B) ImageJ software was used to further quantify the gray values of the western blot bands. (C) The expression of the Nrf2, HO-1, NQO1, and Gpx4 was detected using RT–qPCR and compared with that of an internal reference gene (GAP). (D) The primary microglial membrane protein CD11b was labeled with Texas Red, and Nrf2 was marked by FITC. (E) The primary microglial membrane protein CD11b was labeled with Texas Red, and HO-1 was marked by FITC. The data are shown as the means ± SD of n = 3 samples/group and at least three independent experiments were performed. The statistical analyses were conducted using the two-way ANOVA. *, p < 0.01 and **, p < 0.01.