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. 1998 Dec;64(12):4782–4788. doi: 10.1128/aem.64.12.4782-4788.1998

FIG. 1.

FIG. 1

Determination of HD-133 cry gene families by both triplex and E-PCR, using total genomic DNA. (A) HD-133 cry1 gene families. Only type-specific primers present in the triplets are mentioned below. Triplets contain the I(+) and I(−) primers described elsewhere (21). All primer sequences used for cry1 screening are identical to those previously described (21) except for the cry1Ae type primer called IAe (Table 1). Lane 1, molecular size markers (in kilobases) (type VI; Boehringer); lane 2, IAa; lane 3, IAb; lane 4, IAc; lane 5, IAd; lane 6, IAe; lane 7, IBa; lane 8, ICa; lane 9, IDa; lane 10, IEa; lane 11, IFa; lane 12, IGa (cry9Aal); lane 13, E-PCR with primers I(+), I(−), IAa, IAb, ICa, and IDa; lane 14, molecular size markers. (B) Other cry gene families. Lane 1, molecular size markers (type VI; Boehringer); lane 2, primers II(+) and II(−); lane 3, primers II(∗), II(−), and IIA; lane 4, primers II(+), II(−), and IIB; lane 5, E-PCR with primers II(+), II(−), and IIB; lane 6, primers III(+) and III(−); lane 7, primers V(+) and V(−); lane 8, oligonucleotides VI(+) and VI(−); lane 9, primers 7/8(+) and 7/8(−); lane 10, molecular size markers.