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Partial nucleotide sequence of the cloned HD-133 chromosomal cry1Aa gene. The 5′ end of the cloned cry1Aa gene is shown from the NdeI site used for cloning into the vector pMPCV up to and including the first 34 nucleotides of cry1Aa, starting at nucleotide 92 (boldface). The directionality of the cry1Aa coding sequence is indicated by the arrow. Immediately upstream of the cry gene sequence is an exact match to the left inverted repeat of IS231 (open box).
