TABLE 1.
Primer sequences used in PCR screening
| Primer namea | Sequenceb | Expected PCR band size (kb) |
|---|---|---|
| I(+) | 5′-TRACRHTDDBDGTATTAGAT-3′ | |
| I(−) | 5′-MDATYTCTAKRTCTTGACTA-3′ | 1,500–1,600 |
| IAe | 5′-CTCTACTTTTTATAGAAACC-3′ | 1,169 |
| II(+) | 5′-TAAAGAAAGTGGGGAGTCTT-3′ | |
| II(−) | 5′-AACTCCATCGTTATTTGTAG-3′ | 1,556 |
| IIA | 5′-TCTCATAGGGGCGACTAATC-3′ | 694 |
| IIB | 5′-TGATATAGGTGCATCTCCGT-3′ | 694 |
| III(+) | 5′-AAACHGAAYTAACAAGAGAC-3′ | |
| III(−) | 5′-AASTKAGWKGTWGAAGCATA-3′ | 858 |
| V(+) | 5′-ATGAAACTAAAGAATCCAGA-3′ | |
| V(−) | 5′-AGGATCCTTGTGTTGAGATA-3′ | 1,137 |
| VI(+) | 5′-TAYGGTTTTAAAKKTGCTGG-3′ | |
| VI(−) | 5′-TRAATYCTATTRAACAATCCTA-3′ | 587 |
| 7/8(+) | 5′-YCRDTTYCGYAGAGARATGA-3′ | |
| 7/8(−) | 5′-YYTCTAAWYCYTGACTACTT-3′ | 1,704 |
a
All primers used in this study but not listed above [except the I(+) and I(−) primers] were described elsewhere (19). Primers 7/8(+) and 7/8(−) were directed against both the cry7 and cry8 genes.
b
Family primers are mostly degenerate in order to recognize all known family sequences. B = C, G, or T; D = A, G, or T; H = A, C, or T; K = G or T; M = A or C; R = A or G; Y = T or C.