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. 2022 Apr 27;13:782684. doi: 10.3389/fphys.2022.782684

FIGURE 2.

FIGURE 2

SIRT1 levels and localization in the neurons of ischemic penumbra 4 and 24 h after photothrombotic stroke in the rat cerebral cortex (PTS4 and PTS24, respectively) compared to the same animals’ contralateral cortex (CL4 and CL24), or the cerebral cortex of sham-operated animals (SO). (A) Immunofluorescence of SIRT1 (green), neuronal marker NeuN (red), nuclear chromatin marker Hoechst 33342 (blue), and image overlay. The scale bar is 100 μm. (B) Percent changes (ΔISO) of SIRT1 levels in the penumbra (PTS4 or PTS24) and the contralateral hemisphere (CL4 or CL24) compared to the brain cortex of the sham-operated rats (SO) 4 or 24 h after the PTS. (C) Percent changes (ΔICL) of SIRT1 levels in the penumbra (PTS4 or PTS24) compared to the contralateral cortex of the same rats 4 or 24 h after PTS. (D) Coefficient M1 of SIRT1 co-localization with the neuronal marker (NeuN) in different control and experimental groups. (E) Coefficient M1 of SIRT1 co-localization with the nuclear marker (Hoechst 33342) in different control and experimental groups. (F) Immunofluorescence of SIRT1 in the penumbra at bigger magnification (×40). The scale bar is 30 μm. One Way ANOVA; M ± SEM; n = 7.