Figure 5.

sEVs^RVG-siRNA protected the pregnant and fetal mice from ZIKV infection by crossing the placental barrier

Pregnant mice received intrauterine injections with ZIKV at E10.5, followed by three injections of sEVs^RVG-siRNA by tail injection, and mice were killed at E19.5 to obtain various tissues for testing. si#3 (si#3 represents siNS1) was packaged into sEVs^Ctrl/sEVs^RVG for mouse experiments. siNC means negative control. (A) Scheme diagram of pregnant mice being treated. (B–J) Viral titer in placenta (B), uterine horn (C), serum (D), brain (E), liver (F), and spleen (G) of the pregnant mice. Viral titer in brain (H), liver (I), spleen (J) of fetuses. Data are expressed as mean ± SD (n = 5–6), and analysis was performed by one-way ANOVA with Bonferroni's multiple comparisons test (∗p < 0.05; ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001). All the data show mean ± SD from three experimental replicates.