FIGURE 6.

ICAM-1^−/− mice produce less NETs in the lungs in sepsis. Sepsis was induced in WT and ICAM-1^−/− mice by cecal ligation and puncture (CLP). After 4 hours of CLP, lung tissues were harvested and single cell suspensions were prepared. Cells were surface-stained with APC-rat anti-mouse Ly-6G Ab, FITC-mouse anti-MPO Ab, and rabbit anti-histone H3 (CitH3) Ab followed by staining with PE-donkey anti-rabbit IgG and NETs were determined by flow cytometry. A, Representative dot plots of the NETs⁺ cells in the lungs are shown. B, The frequencies and (C) numbers of NET forming neutrophils in lungs are shown. Experiments were repeated at least three times. Data are expressed as mean ± SE (n = 6 mice/group). The groups were compared by one-way ANOVA and SNK method (*P < .05 vs WT sham; ^#P < .05 vs WT CLP)