Fig. 1.

TIM is a conserved phosphorylation site in mTOR-dependent AGC kinases. (A) Crystal structure of the isolated kinase domain of protein kinase CβII (2I0E) showing the conserved C-terminal tail (C-tail; turquoise). The C-tail phosphorylation sites are indicated: TIM (pink; Thr634 in PKCβII), turn motif (orange; Thr641 in PKCβII), and hydrophobic motif (green; Ser660 in PKCβII). Sequence logos display conserved features of the AGC C-tail for all mammalian kinases containing the TIM Thr (F-x3-F-pT), including a conserved PXXP motif (Kannan et al., 2007). (B) AGC branch of the human kinome tree, with red highlighting indicating kinases with the TOR interaction motif (Metz et al., 2018). Adapted from Baffi et al. (2021a). Reprinted with permission from American Association for the Advancement of Science.