REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Human BV421-CD34 (clone 561) 1:20	BioLegend	Cat#343609, RRID: AB_11147951
Human FITC-Lineage mix (CD3/14/19/20/56) 1:20	BioLegend	Cat#348701, RRID: AB_10644012
Human PE-CD38 (clone HIT2) 1:50	BioLegend	Cat#303505, RRID: AB_314357
Human APC-CD90 (clone 5E10) 1:200	BioLegend	Cat#328113, RRID: AB_893440
Human PerCP/CY5.5-CD45RA (clone HI100) 1:20	BioLegend	Cat#304121, RRID: AB_893358
Human Pe/Cy7-CD49f (Clone GoH3) 1:100	BioLegend	Cat#313621, RRID: AB_2561704
Human FITC-CD16 (clone 3G8) 1:100	BioLegend	Cat#302005, RRID: AB_314205
Human FITC-CD11c (clone 3.9) 1:20	BioLegend	Cat#301603, RRID: AB_314173
Chemicals, peptides, and recombinant proteins
Human Stem Cell Factor (SCF)	Miltenyi Biotec	Cat#130-096-696
Human Flt3 – Ligand	Miltenyi Biotec	Cat#130-096-480
Interleukin-6 (IL-6)	Stem Cell Technologies	Cat#78050
Interleukin-3 (IL-3)	Stem Cell Technologies	Cat#78040
Thrombopoietin (TPO)	Miltenyi Biotec	Cat#130-095-754
Primocin	InvivoGen	Cat#ant-pm-05
UM729	Stem Cell Technologies	Cat#72332
Stemregenin-1 (SR1)	Stem Cell Technologies	Cat#72344
Critical commercial assays
CD34 Microbead Kit UltraPure, human	Miltenyi Biotec	Cat#130-100-453
LS Columns	Miltenyi Biotec	Cat#130-042-401
QIAamp DNA Micro Kit	QIAGEN	Cat#56304
TruSeq DNA Nano	Illumina	Cat#20015964
Software and algorithms
Nextflow Illumina Analysis Pipeline (NF-IAP)		https://github.com/ToolsVanBox/NF-IAP Version 1.3.0
GRIDSS-PURPLE-LINX standalone pipeline		https://github.com/hartwigmedical/gridss-purple-linx
Somatic Mutations Rechecker and Filtering (SMuRF)		https://github.com/ToolsVanBox/SMuRF Version > 2.0.0
R		https://www.r-project.org/ Version > 4.0.0
R package: MutationalPatterns		https://bioconductor.org/packages/release/bioc/html/MutationalPatterns.html Version > 3.0.0
R package: drc: Analysis of Dose-Response Curves		https://cran.r-project.org/web/packages/drc/index.html Version > 3.0.0
Supplementary code for displaying mutation contexts and mutation blacklists		https://github.com/ToolsVanBox/Genotoxin_assay
Other
50 mL LeucoSep tube with porous insert, pre-filled with Leucosep separation medium	Greiner	Cat#227288
Lymphoprep	Stem Cell Technologies	Cat#07861
StemSpan SFEM I Medium	Stem Cell Technologies	Cat#09650
Iscove’s Modified Dulbecco’s Medium (IMDM)	Thermo Fisher Scientific	Cat#12440053
CELLSTAR plate, 384w, 130 μL, F-bottom, TC, cover	Greiner Bio-One	Cat#781182
Dimethyl sulfoxide (DMSO)	Sigma-Aldrich	Cat#D8418, CAS: 67-68-5
Fetal bovine serum (FBS)	Thermo Fisher Scientific	Cat#10500
Bovine serum albumin (BSA)	Sigma-Aldrich	Cat#A9647-50G, CAS: 9048-46-8
Ethylenediaminetetraacetic acid (EDTA)	Sigma-Aldrich	Cat#E4884-500G, CAS: 6381-92-6
DNA LoBind 1.5 mL tubes	Eppendorf	Cat#022431021
Trypan Blue	Sigma-Aldrich	Cat#T8154, CAS: 72-57-1
Hemocytometer	Marienfeld	Cat#0640311
DNase I, Bovine Pancreas	Merck/Millipore	Cat#260913, CAS: 9003-98-9